We are a CRO with expertise in mitochondrial respirometry for clinical, translational, and basic science. We support clinical trials studying the effects of disease and therapeutics on mitochondrial function.
Our clients are biotechnology and pharmaceutical companies, as well as academic institutions.
- Permeabilized Cell Respiration
- ROS Production
- Metabolic Substrate Utilization
- Intact Cell Respiration
- Compound / Drug Screen
Additional Mitochondrial Assays
Mitochondrial Complex Quantification (Western Blot)
- Mitochondrial Content (Western Blot)
- Citrate Synthase Activity
- Cell Culture
- Blood Cell Isolation
- Study Design
- Data Analysis and Interpretation
We’re more than just a contract research organization. Our staff and collaborators are actively engaged in advancing mitochondrial science through their independent academic research. Our clients gain access to thought leaders in the field with decades of experience. We deliver insight beyond the ordinary.
When mitochondrial analysis is critical to your mission, our focused expertise makes Aeva your partner of choice.
Mitochondrial Function Overview
Mitochondria produce the majority of a cell’s energy, and nearly all cells have them.
The mitochondrion is comprised of an inner (IM) and outer membrane (OM). The inner membrane contains many folds, called cristae, to increase the surface area of the matrix, where most substrate metabolism occurs.
Metabolic substrates are processed by the Tricarboxylic Acid (TCA) cycle to produce NADH which is used by Complex I of the Electron transport chain (ETC). Together, Complex I and II, along with the Electron-transferring flavoprotein complex (CETF) and the mitochondrial glycerol-3-phosphate dehydrogenase (mGpDH), pass electrons to ubiquinone (Q), Complex III, and finally Complex IV, where four electrons reduce oxygen into water.
A proton gradient is also generated by complexes I, III, and IV, between the matrix and the inner membrane space (IMS). At complex V, this proton-motive force drives the phosphorylation of ADP into ATP, the molecule most commonly used to power enzymatic functions throughout the cell.
Facilities & Equipment
Aeva operates out of Innovation Quarter in downtown Winston-Salem, North Carolina, home to the old Bailey Power plant. A location appropriate for mitochondrial research given the common saying Mitochondria are the powerhouse of the cell.
“The mitochondrion, like any power plant, utilizes fuel supplies and intact power conduits to generate the energy that keeps our clocks ticking.”
Philip Kramer, PhD
Chief Science Officer
- Intact and permeabilized cells and tissues
- Unlimited injections
- Low Throughput
- High resolution
Fluorometric / Optical Probes
- Intact cells
- Four injection ports
- High throughput
- Low resolution